Özet:
New knot configurations, consisting of alternating strands with different patterns, have been studied from mechanical and biological perspectives in order to determine whether they would be suitable for abdominal surgery, as compared with conventional sliding knots. Mechanical properties of these new knots were compared with those of the classical sliding knots and single threads for silk and nylon sutures under dry conditions. From the mechanical perspective, the new knots showed better knot holding capacity and efficiency. In the in vivo implantation tests performed on the rat abdominal wall, the alternating sliding knots with different patterns were found to be more efficient and secure than the classical sliding knots. The knot configuration, postoperative period, suture material and size were important factors in determining the knot holding capacity. From the biological perspective, these new knots provoked tissue reaction similar to the classical sliding knots. Because nylon is less pliable than silk, its use resulted in higher effective knot volumes, causing more pronounced tissue reaction. To test the bacterial adherence to the knots, in vitro and in vivo tests were performed in rats. The degree of the elicited infection correlated well with the capability of bacteria to bind to the suture. It was observed that the knot configurations and the suture sizes did not have much effect on bacterial adherence. Due to the presence of interstices between throws, the knots had greater capacity to retain bacteria than the single threads for both silk and nylon, thus promoting infection. The elasticity and stress-relaxation properties of these knots were compared to those of single threads of silk and nylon. The elasticity of the knots, in general, was higher than that of the threads for both materials. The silk showed decreased elasticity at high extension levels, while nylon showed increased elasticity. In stress relaxation tests, the residual load fraction of the knots was found to be higher than that of the threads at all extension levels. A model was created to study the effect of several factors on the suture pullout force in the abdominal wall. Incisional direction, knot configuration, strain rate and tissue healing strength were important factors in determining the suture pullout force. In conclusion, we do recommend the use of the alternating sliding knots with different patterns in abdominal surgery instead of the currently used sliding knots.
