Bone surface mimicked biodegradable polymeric scaffolds

Abstract

In this thesis, bone surface topography was mimicked by a using biodegradable polymer. In the rst part, bone surface topography was mimicked and transferred to the polydimethylsiloxane (PDMS) surface using soft lithography technique. Bone surface mimicked Polylactic acid (BSM-PLA) prepared by solvent casting using the PDMS as a mold. The e ect of PLA concentration (2.5-10% (w/v) in chloroform) and casting time (as evaporated-24 h) were investigated to obtain best mimicking conditions. After characterization of BSM-PLA sca olds by scanning electron microscopy (SEM), the best mimicked sca olds were obtained at 10% PLA concentration and 24 h casting time. The e ectiveness of bone mimicking procedure was also investigated by SEM. As a result, same bone and PDMS surface could be used several times to fabricate BSM-PLA sca olds. The fabricated BSM-PLA sca olds' surface characterization results showed that the fabricated BSM-PLA surface hydrophobicity and roughness were improved to guide cells attachment. In second part, in-vitro degradation in terms of weight loss and morphology, and cumulative drug release tests were performed. Compared with the BSM-PLA, plain PLA sca olds degraded more rapidly in phosphate bu er solution (PBS). The result was the same for the rate of drug release pro le in PBS as well. For the last part of the thesis, the e ect of surface topography on human bone marrow mesenchymal stem cells (hBM MSCs) viability and di erentiation were investigated using BSM-PLA sca olds. According to these results, stem cell incorporation onto BSM sca olds as a future trend is addressed shortly highlighting the immense potential for osteogenic stem cell di erentiation that features high adaptiveness to the biological environment. Consequently, the developed BSM-PLA sca olds are predicted to have a great potential on the surface 3D sca olds fabrication and guidance of stem cells that are provided for bone tissue engineering applications.|Keywords : Biomimetic, Bone tissue engineering, Stem cells.