Low level laser therapy on human adipose tissue derived mesenchymal stem cells

Abstract

Recent in vitro studies on cell cultures provided that low level lasers have various biostimulatory effects on several tissues. Biostimulation of tissues and cells is an important issue in Tissue Engineering Applications like regeneration from stem cells. Therapeutic potential of mesenchymal stem cells (MSCs) from bone marrow or umbilical cord are now being tested for many lethal and chronic disorders worldwide; however the clinical use of these MSCs has presented problems. Current results indicate that adipose tissue can be a novel and abundant source for adult MSCs. In this study, the effects of 650 nm and 635 nm diode laser on proliferatıon of human adipose tissue derived mesenchymal stem cells were examined. Two different dosages (1,6 J/cm2 and 3,2 J/cm2) were applied for both two wavelengths. 24 h after seeding, irradiation was started. Cells were exposed to laser irradiation for three consecutive days. Cells were counted at 5th, 9th, 14th and 21st days after seeding. It was observed that the extent of enhancement of the cell proliferation by 650 nm diode laser was significantly higher relative to control group at 14th day. 635 nm diode laser application results showed significantly higher growing enhancement relative to control group on 5th day.|Keywords: Low Level Laser Therapy, Biostimulation, Mesenchymal Stem Cell, Cell Proliferation.