Arşiv ve Dokümantasyon Merkezi
Dijital Arşivi

Identification and docking analysis of allatostatin receptor in Carausius morosus

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dc.contributor Graduate Program in Molecular Biology and Genetics.
dc.contributor.advisor İyison, Necla Birgül.
dc.contributor.author Duan, Burçin.
dc.date.accessioned 2023-03-16T11:26:09Z
dc.date.available 2023-03-16T11:26:09Z
dc.date.issued 2012.
dc.identifier.other BIO 2012 D83
dc.identifier.uri http://digitalarchive.boun.edu.tr/handle/123456789/15452
dc.description.abstract Allatostatin receptors (AlstRs) with their ligands (ASTs) play role in insect development, by inhibiting the production of Juvenile hormone. This thesis project uses the advances in structure-based studies and single-molecule based approaches in order to obtain a detailed information about AlstR-AST interaction in a model laboratory organism Carausius morosus. The mRNA sequence of AlstR was first obtained via 5' and 3' RACE reactions with the help of speci c primers designed on conserved transmembrane region previously found by Birgül et al. The longest ORF was predicted and identi ed as an Allatostatin Receptor type C of insects. As a result it was named as AlstR-C of C. morosus (CamAlstR-C) in this project. Then, evolutionary analysis was combined with bioinformatics modeling and docking tools, to obtain some features of CamAsltR-C interaction pattern. This interaction was veri ed in vivo via atomic force microscopy. This was the rst time of AFM study on living cells, in Bo gazi ci University. Binding force was detected in CamAlstR-C over-expressed cells. In addition, the binding pocket was predicted bioinformatically, to be composed of second, third and N-terminal extracellular loops of CamAlstR-C. And in more detail, a speci c conserved binding motif (IXTXP) was proposed on the third extracellular loop, which may be identical in other AlstRs.
dc.format.extent 30 cm.
dc.publisher Thesis (M.S.) - Bogazici University. Institute for Graduate Studies in Science and Engineering, 2012.
dc.relation Includes appendices.
dc.relation Includes appendices.
dc.subject.lcsh Carausius morosus.
dc.subject.lcsh Juvenile hormones.
dc.title Identification and docking analysis of allatostatin receptor in Carausius morosus
dc.format.pages xx, 99 leaves ;


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