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Fabrication of thiol reactive polymeric microstructures

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dc.contributor Graduate Program in Chemistry.
dc.contributor.advisor Sanyal, Amitav.
dc.contributor.author Gevrek, Tuğçe Nihal.
dc.date.accessioned 2023-03-16T11:01:44Z
dc.date.available 2023-03-16T11:01:44Z
dc.date.issued 2011.
dc.identifier.other CHEM 2011 G48
dc.identifier.uri http://digitalarchive.boun.edu.tr/handle/123456789/14485
dc.description.abstract PEG-based 3D hydrogel patterns containing thiol reactive maleimide functional groups have been prepared using a novel Diels-Alder/retro Diels-Alder strategy. Bulk-hydrogels and hydrogel patterns containing various amount of thiol reactive maleimide were synthesized by photo-polymerization in presence of photoinitiator and PEG-DA cross linker. The thiol reactive maleimide group was protected with furan during gelation of bulk-hydrogel and patterning of hydrogels and then was deprotected by retro-Diels-Alder reaction. After activation of maleimide in the hydrogel structure, the reactive maleimide group embedded hydrogels can be efficiently derivatized with thiol containing molecules such as a fluorescent dye, BodipyC10SH. Thiol containing biotin derivatives were covalently attached to the thiol reactive maleimide in bulk-hydrogel and hydrogel patterns under mild condition. Immobilization of FITC-streptavidin onto biotinylated hydrogel patterns were performed and investigated by fluorescence microscope. In order to detect amount of accessible biotin in the hydrogel, HABA-assay was carried out with biotinylated bulk-hydrogels.
dc.format.extent 30 cm.
dc.publisher Thesis (M.S.)-Bogazici University. Institute for Graduate Studies in Science and Engineering, 2011.
dc.relation Includes appendices.
dc.relation Includes appendices.
dc.subject.lcsh Thiols.
dc.subject.lcsh Reactive polymers.
dc.title Fabrication of thiol reactive polymeric microstructures
dc.format.pages xii, 44 leaves ;


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