Bilgen, Bahar.
(Thesis (M.S.)- Bogazici University. Institute for Graduate Studies in Science and Engineering, 2000, 2000.)
The gene encoding the Taq I restriction endonuclease was cloned into pMAL-c2 plasmid to construct p22 expressing the MBP-Taq I fusion protein. The affinity of MBP to amylose was exploited for the single-step chromatographic ...