Glucose isomerase (GI), which catalyzes the reversible isomerization of Glucose toFructose, is of commercial importance in the production of high fructose corn syrup. Athigh temperature, equilibrium for the isomerisation ...

A small scale procedure developed for the purification of EcoRI restriction enzyme has been applied to two different overproducing E.coli strains. The final purification yields obtained are 1.3x105 U/g cells for E.coli ...

The gene encoding the Taq I restriction endonuclease was cloned into pMAL-c2 plasmid to construct p22 expressing the MBP-Taq I fusion protein. The affinity of MBP to amylose was exploited for the single-step chromatographic ...